Cloning of aBacteroides fragilischromosomal determinant coding for 5-nitroimidazole resistance
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Cloning a gene coding for norflurazon resistance in cyanobacteria.
The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experim...
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One of the most efficient mechanisms by which plants protect themselves from invading virusesis the specific RNA-dependent silencing pathway termed post-transcriptional gene silencing(PTGS). In this mechanism, resistance to a virus is engineered through the expression of asegment of the virus genomein transgenic plants. Potato VirusY (PVY) is one of the mostdamaging viruses of potato, infecting...
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OBJECTIVES The mechanism of action of, and resistance to, metronidazole in the anaerobic (or micro-aerotolerant) protozoan parasite Giardia lamblia has long been associated with the reduction of ferredoxin (Fd) by the enzyme pyruvate:ferredoxin oxidoreductase (PFOR) and the subsequent activation of metronidazole by Fd to toxic radical species. Resistance to metronidazole has been associated wit...
متن کاملPlasmids pIP419 and pIP421 from Bacteroides: 5-nitroimidazole resistance genes and their upstream insertion sequence elements.
The genetic organization of two different 5-nitroimidazole (5-Ni) resistance genes was investigated: nimC and nimD from Bacteroides plasmids pIP419 and pIP421, respectively. The nimC gene (492 bp) and the nimD gene (495 bp) directed the synthesis of polypeptides with deduced molecular masses of 18.37 kDa and 18.48 kDa, respectively. The predicted proteins showed 67-83% identity and 78-91% simil...
متن کاملIn-vitro studies of two 5-nitroimidazole derivatives.
This paper reports the findings obtained using two new compounds belonging to the 5-nitroimidazole family: sulphuridazole (V1) and sulphonidazole (V2). We first assessed their antimicrobial activity on Clostridia spp. and then extended the study to Gram-positive and Gram-negative aerobic microorganisms and to Candida albicans. Their MICs were compared with those of metronidazole. The findings s...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1992
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1992.tb05334.x